The status of the rat as a experimental animal in the study of gastric secretion is unique, since a continous secretion . The lower order secretion isnot clear. (Mn ghosh, 6)
For the study of gastric secretion, animals are always fasted (water allowed) for a period varying from 24 to 72 hours. Special care should be taken so that donnot get access to their stools, because they sr ein the habit of estimg their own stools (coprophagy) (mn ghosh, 6)
Sacrifice part
Check the ph of the content of the fluid of stomach
Stomach get incission and hold like the above and determine the number of the grading
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Determination of acidity, free and total Topfer Reagent
Filter the contents of sample through two thicknesses of gauze, if necessary. Take 10 ml of it and put it in a porcelain evaporating dish and add 1 or 2 drops Topfer Reagent and 1 or 2 drops of phenolphthalein solution (1% alcoholic solution). Titrate with 0.1 N NaOH until the red colour disappears. This reading should be taken for free hydrochloric acid. Continue titration until the red colour of phenolphthalein appears, further addition of alkali does not darken the red colour. Take the burette reading for the total acidity, counting from the original reading.